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Be aware: It is best to try freezing the cells at many concentrations to determine which concentration offers the specified viability, recovery, and features on thawing.
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Cryopreservation is often a strategy of employing low temperatures to preserve cells and tissues for foreseeable future use. This system consists of cooling cells to incredibly lower temperatures (-eighty˚C to -196˚C) and suspending their cellular metabolism, which preserves the cells for an indefinite period of time.
Focus of cells inside of a vial: The exceptional concentration for freezing cells may possibly range according to your cell type. Even though freezing cell suspensions at a very lower focus could lead on to minimal cell viability after thawing1, an incredibly high concentration could lead on to undesirable cell clumping.
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Distinct cells, based upon their biological make-up, may well respond in a different way to a provided cryopreservation protocol, that may impact put up-thaw viability. This warrants the necessity for creating an optimized protocol to your cell style of interest.
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